The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The specificity for the detection of low-copy targets is enhanced.
The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer (Patent pending) in the master mix.
High specificity and robust enzyme enable the detection of a broad dynamic range.
The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type) including ABI, Bio-Rad, Roche, and TaKaRa. The 50x ROX reference dye in the kit can be applied to real-time cyclers that require a passive reference dye.