biotechrabbit™ 1-Step RT-PCR Master Mix provides an easy and efficient way to perform both reverse transcription of RNA and PCR amplification of cDNA in one step. Only RNA template, primers and PCR-grade water are added.
The 20× RT-RI Blend, which contains a blend of an efficient thermostable reverse transcriptase and a proprietary Ribonuclease Inhibitor, ensures high yields of cDNA.
The 2X 1-Step Master Mix contains unique Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers in an optimized buffer and provides high PCR product yields with minimal background even when using low-abundance and difficult templates. PCR enhancers included in the mix allow efficient amplification of complex templates including GC or AT-rich sequences.