biotechrabbit CAPITAL qPCR Green Mix allows sensitive and specific amplification with an excellent signal to noise ratio and rapid extension rates. Extremely low-copy-number targets can be detected with high efficiency over several logs of template concentration, while primer-dimer formation is efficiently minimized.
CAPITAL qPCR Green Mix shows accurate and robust performance in wide range of applications, including gene expression and copy number variation analysis.
To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three kit formats are available: a kit containing no ROX, as well as LRox and HRox versions containing ROX in the corresponding concentrations.
Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).