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biotechrabbit GenUP™ Exo SAP Kit

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MT-CM-BR0701800 / (10 reactions sample)
MT-CM-BR0701801 / (100 reactions)
MT-CM-BR0701802 / (500 reactions)
MT-CM-BR0701803 / (2000 reactions)


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    • Fast 15 min PCR cleanup without the need for spin columns
    • 100% recovery — no loss of PCR product
    • Simple process — just add Exonuclease 1 and Shrimp Alkaline Phosphatase to PCR mix
    • Easy scalability and automation

biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.

The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.

Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' → 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.


    • PCR cleanup prior to sequencing or genotyping
    • Removal of nucleotides and primers from PCR reaction
  •  QPS-101T
    • THUNDERBIRD® Probe qPCR Mix    1 ml × 1
    • 50x ROX reference dye    50 µl × 1
  •  
  • QPS-101
    • THUNDERBIRD® Probe qPCR Mix    1.67 ml × 3
    • 50x ROX reference dye    250 µl × 1
  •  
  • QPS-101X5
    • THUNDERBIRD® Probe qPCR Mix    (1.67 ml × 3) x 5
    • 50x ROX reference dye    250 µl × 5




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye




  • Hot Start
  • Probe
  • 2x Reaction mix
  • 20µl total reaction volume
  • Store at -20oC
  • 50x ROX reference dye