The RTS 100 E. coli LinTempGen Set is designed to rapidly generate linear expression constructs that are ready for use with RTS 100 E. coli HY, Fab and insect kits. PCR generated linear templates allow fast protein expression from wild type or modified DNA sequences. Use PCR-based template generation to:
- Add epitope-tag sequences for antibody detection
- Introduce mutations
- Change codon usage
- Generate truncated proteins
Using linear DNA templates for protein expression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression. Gene-specific primers can be used for RTS Wheat Germ and E. coli LinTempGen sets enabling easy transfer of products from any expression system to both the RTS Wheat Germ and E. coli platforms. For expression scale-up, the linear templates can be cloned into RTS pIVEX E. coli vectors and used with RTS 100/500/9000 E. coli CECF kits.
(former product codes: RiNA N-3510-96, 5PRIME 2401000)
