The RTS Wheat Germ LinTempGen Set, His6-tag can be used to rapidly generate linear expression constructs that are ready for use with RTS Wheat Germ kits. PCR generated linear templates allow fast protein expression from wild type or modified DNA sequences. Use PCR-based template generation to:
- Add epitope-tag sequences for antibody detection
- Introduce mutations
- Change codon usage
- Generate truncated proteins
Using linear templates for protein expression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression. Gene-specific primers can be used for RTS Wheat Germ and E. coli LinTempGen sets enabling easy transfer of products from any expression system to both the RTS Wheat Germ and E. coli platforms. For expression scale-up, the linear templates can be cloned into the RTS pIVEX Wheat Germ vector and used with RTS 100/500 Wheat Germ CECF kits.
(former product codes: RiNA N-3540-96, 5PRIME 2402000)
