Merck Methanol

Product Name:  Methanol, gradient grade for liquid chromatography LiChrosolv® Reag. Ph Eur

Grade:  gradient grade

             gradient grade

Quality Level:  200

Agency:  reag. Ph. Eur.

vapor density:  1.11 (vs air)

vapor pressure:  128 hPa ( 20 °C)

                           410 mmHg ( 50 °C)

                           97.68 mmHg ( 20 °C)

product line:  LiChrosolv®

Assay:  ≥99.9% (GC)

Form:  liquid

autoignition temp.:  725 °F

potency:   5628 mg/kg LD50, oral (Rat)

                 17100 mg/kg LD50, skin (Rabbit)

expl. lim.:  36 %

technique(s):  HPLC: suitable

impurities:  ≤0.0002 meq/g Acidity

                   ≤0.0002 meq/g Alkalinity

                   ≤0.02% Water

evapn. Residue:  ≤2.0 mg/L

color:  APHA: ≤10

transmittance:

210 nm, ≥20%

220 nm, ≥60%

230 nm, ≥75%

235 nm, ≥83%

250 nm, ≥95%

260 nm, ≥98%

refractive index:  n20/D 1.329 (lit.)

kinematic viscosity:  0.54-0.59 cSt(20 °C)

bp:  64.7 °C (lit.)

mp:  −98 °C (lit.)

transition temp:  flash point 9.7 °C

density:  0.791 g/mL at 25 °C (lit.)

UV absorption:  λ: 225 nm Amax: ≤0.170

Format:  neat

storage temp.:  2-30°C

SMILES string:  CO

InChI:  1S/CH4O/c1-2/h2H,1H3

InChI key:   OKKJLVBELUTLKV-UHFFFAOYSA-N

General description

With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in "gradient grade" as well as "isocratic grade". This enables to minimize the gradient effect of the solvent involved.

Application

Methanol is used as a solvent:

• In the mobile phase to separate mercury species on a Zorbax C18 reverse phase column. [1]
• To wash the cartridges and as an eluent to extract the analytes during the solid-phase extraction (SPE) process. [2]

Preparation Note

Product filtered through a 0.2 μm filter

Analysis Note

Evaporation Number: 1.9

Purity (GC): ≥ 99.9 %

Identity (IR): conforms

Evaporation residue: ≤ 2.0 mg/l

Water: ≤ 0.02 %

Color: ≤ 10 Hazen

Density (d 20 °C/20 °C): 0.791 - 0.793

Boiling point: 64 - 65 °C

Acidity: ≤ 0.0002 meq/g

Alkalinity: ≤ 0.0002 meq/g

Gradient grade (at 235 nm): ≤ 2.0 mAU

Gradient grade (at 254 nm): ≤ 1.0 mAU

Fluorescence (as quinine at 254 nm): ≤ 1.0 ppb

Fluorescence (as quinine at 365 nm): ≤ 0.5 ppb

Transmission (at 210 nm): ≥ 20 %

Transmission (at 220 nm): ≥ 60 %

Transmission (at 230 nm): ≥ 75 %

Transmission (at 235 nm): ≥ 83 %

Transmission (at 250 nm): ≥ 95 %

Transmission (from 260 nm): ≥ 98 %

Absorbance (at 225 nm): ≤ 0.170

Filtered by 0.2 µm filter.

Suitable for UPLC / UHPLC /Ultra HPLC - instruments.

Conforms to Methanol R1 and R2 according to Reag. Ph Eur;

conforms to the requirements of ACS for liquid chromatography suitability.

Other Notes

Explore various lab safety accessories and equipment for safe handling of solvents to increase your safety level from the first usage.

Legal Information

LICHROSOLV is a registered trademark of Merck KGaA, Darmstadt, Germany