Merck Sodium dodecyl sulfate

biological source:  synthetic

Quality Level:  200

Grade:

Molecular Biology

for molecular biology

Description:  anionic

product line:  BioReagent

Assay:  ≥98.5% (GC)

Form:  powder

mol wt:  micellar 18,000

aggregation number:  62

technique(s):  electrophoresis: suitable

impurities:  ≤2.0% water (Karl Fischer)

CMC:  7-10 mM (20-25°C)

Mp:  204-207 °C (lit.)

transition temp:  cloud point >100 °C

solubility:  water: soluble

density:  1.03 g/cm3 at 20 °C (68 °F)

anion traces:

chloride (Cl-): ≤500 ppm

phosphate (PO43-): ≤10 ppm

cation traces:  heavy metals (as Pb): ≤10 ppm

absorption:

≤0.1 at 260 at 3%

≤0.1 at 280 at 3%

HLB:  40

Suitability:  suitable for electrophoresis

foreign activity:  DNase, RNase, none detected

storage temp.: room temp

SMILES string:  [Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI:  1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key:  DBMJMQXJHONAFJ-UHFFFAOYSA-M

General description

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is an anionic surfactant widely used in various scientific applications, particularly in molecular biology, biochemical, and cell biology research. Its effectiveness stems from its amphiphilic nature, allowing it to interact with both hydrophobic and hydrophilic molecules. SDS is a versatile detergent that excels in solubilizing and denaturing proteins, making it an essential tool in protein extraction and purification procedures. By disrupting protein structures, SDS facilitates the separation of proteins based on their molecular weight during SDS-PAGE electrophoresis.

Beyond protein analysis, SDS also finds applications in the electrophoretic separation of lipids, enabling the study and characterization of these important cellular components. Additionally, SDS is employed in various hybridization and molecular biology techniques, demonstrating its versatility across a range of research areas. SDS′s effectiveness in both ionic and anionic conditions further expands its utility, allowing it to adapt to diverse experimental setups and requirements. Its ability to unravel proteins by binding to peptide chains and imparting a negative charge effectively eliminates protein shape as a factor for gel separation, ensuring accurate protein size determination.

Application

Anionic detergent

Sodium dodecyl sulfate has been used:

• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Features and Benefits

• Highly versatile surfactant for your molecular biology and biochemical research
• Suitable for electrophoresis applications
• Tested to confirm low levels of heavy metal contamination,ensuring suitability for various applications.

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Other Notes

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