General description
25 kDa
Chromatin assembly factor-1 (CAF-1) is a multisubunit
protein complex that comprises three polypeptide subunits known as p150, p60,
and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized
and acetylated histones H3/H4 into nascent chromatin during DNA replication.
The p150 subunit of CAF-1 also supports the maintenance of heterochromatin,
which requires the synthesis of both new histones and heterochromatin proteins
and their orderly assembly during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally
replicates late during S phase, has a low gene density, and contains large
blocks of repetitive DNA that is relatively inaccessible to DNA-modifying
reagents. In late S phase, p150 directly associates with heterochromatin
associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis,
CAF-1 p150 and some HP1 progressively dissociate from heterochromatin,
coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate
with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Immunogen
GST fusion protein corresponding to residues 17-173 of human
HP1γ protein. Clone 42s2.
Application
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect HP1γ
positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to
immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked
chromatin.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal
Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as
Heterochromatin protein 1 homolog gamma.
Biochem/physiol Actions
Reactivity with other species has not been determined.
This antibody recognizes HP1γ protein, Mr ~25 kDa.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.014 M
phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
Liquid at -20ºC.
Preparation Note
Stable for 1 year at -20°C from date of receipt. For maximum
recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
HeLa acid extracts, HEK 293 nuclear extracts.
Routinely evaluated by western blot in acid-extracted HeLa
cell lysates (Catalog #13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell
lysates (Catalog # 13-112).
Other Notes
Concentration: Please refer to the Certificate of Analysis
for the lot-specific concentration.
Due to license agreement restrictions, this product cannot
be purchased for resale.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt,
Germany
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
