Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL
method
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human TPO (P07202-1)
extracellular domain (Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr) was fused with a
polyhistidine tag at the C-terminus.
Accession#
P07202-1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Cys 15
Molecule Mass
The secreted recombinant human TPO comprises 842 amino acids
and has a predicted molecular mass of 93.8 kDa. The apparent molecular
mass of rh TPO (1-846) is approximately 90-100 kDa in SDS-PAGE under reducing
conditions.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10%
gly
Please contact
us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as
protectants before lyophilization.
Please refer to the specific buffer information in the
hardcopy of datasheet or the lot-specific COA.
Shipping
In general, recombinant proteins are provided as lyophilized
powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are
shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of
receipt at -20℃ to -80℃. Store it under sterile
conditions at -20℃ to -80℃. It is recommended that the
protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
To find reconstitution info, click on 'COA' at the top of
the page, enter the lot number and product size for the lot-specific COA.
Human Insulin, Solution is a protein preparation used
extensively in biochemical and physiological research. It is employed in
studies exploring insulin signaling pathways and glucose metabolism, providing
insights into the regulation of blood sugar levels and energy homeostasis.
Researchers use Human Insulin, Solution to stimulate insulin receptors in cell
cultures and to investigate insulin′s role in cellular processes such as
glycogen synthesis, lipid metabolism, and protein synthesis. In diabetes research,
this solution is applied to understand the mechanisms of insulin resistance and
to explore the function of pancreatic beta cells. Additionally, Human Insulin,
Solution is used in assays to assess the insulin sensitivity of various tissues
and to study the downstream effects of insulin binding on target cells.
