General description
Goat anti-rabbit IgG-peroxidase conjugate associates with
all rabbit Igs. The conjugate shows no reaction with human IgG by
immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human
IgG to reduce background staining with human samples.
Immunoglobulin G (IgG) belongs to the immunoglobulin family
and is a widely expressed serum antibody. An immunoglobulin has two heavy
chains and two light chains connected by a disulfide bond. It is a glycoprotein
and mainly helps in immune defense.[1] IgG is a major class of
immunoglobulin.[2] Immunoglobulin G (IgG) participates in hypersensitivity
type II and type III. Rabbit IgG is further divided into five classes- IgM,
IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy
disulfide bridge.[2] It helps in opsonization, complement fixation and
antibody dependent cell mediated cytotoxicity.[3]
Immunogen
Purified rabbit IgG
Application
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has
been used in western blotting , immunofluorescence staining , immunochemistry
and immunoprecipitations.
Co-immunoprecipation and western blot analysis of C33A cell
lysates were performed using HRP conjugated goat anti-rabbit IgG as the
secondary antibody.
Goat anti-rabbit IgG conjugated to peroxidase can be used
for direct ELISA and immunohistochemistry.
Peroxidase-conjugated goat anti-rabbit IgG antibody was used
for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast
lysates following protein carbonylation assays at a dilution of 1:5000.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been
used as a secondary antibody during immunofluorescence staining.
Biochem/physiol Actions
IgGs (immunoglobulins) modulate several immune responses.It
mainly helps in immune defense.[1] Immunoglobulin G (IgG) participates in
hypersensitivity type II and type III. It helps in opsonization, complement
fixation and antibody dependent cell mediated cytotoxicity.[3]
Specificity of the Anti-Rabbit IgG-Peroxidase is determined
by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The
conjugate shows no reaction with human IgG by IEP.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4,
containing 0.05% MIT
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For
extended storage, the solution may be frozen in working aliquots. Repeated
freezing and thawing, or storage in "frost-free" freezers, is not
recommended. If slight turbidity occurs upon prolonged storage, clarify the
solution by centrifugation before use.
Prepared by two-step glutaraldehyde method.
Other Notes
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Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
