General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family
and is a widely expressed serum antibody. Each immunoglobulin has two heavy
chains and two light chains connected by a disulfide bond. It is a
glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five
immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further
divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in
opsonization, complement fixation and antibody dependent cell mediated
cytotoxicity.
Application
Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in
goat has been used in:
- immunohistochemistry
- enzyme-linked
immunosorbent assay (ELISA)
- immunoblot
- dot-blot
analysis
Specificity of a new antibody for PND in blood from
immunized mice was tested by Elisa using biotynlated PND and streptavidin
coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as
secondary.
Biochem/physiol Actions
Goat anti-mouse IgG(Fc specific)-peroxidase antibody is
specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no
reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain,
bovine IgG and IgM, or horse IgG.
Immunoglobulin G (IgG) is a glycoprotein antibody that
regulates immune responses such as phagocytosis and is also involved in the
development of autoimmune diseases. Mouse IgGs have four distinct isotypes,
namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in
mice.
Immunoglobulin G (IgG) participates in hypersensitivity type
II and type III reactions. IgG helps in opsonization and antibody dependent
cell mediated cytotoxicity.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4,
containing 0.05% MIT.
Preparation Note
Adsorbed to reduce background staining with bovine, horse,
or human samples.
Prepared by the two-step glutaraldehyde method described by
Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Other Notes
2024
CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Antibody adsorbed with bovine, equine and human serum
proteins.
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
