biological source:
goat
Quality Level: 200
Conjugate: peroxidase
conjugate
antibody form: affinity
isolated antibody
antibody product type:
secondary antibodies
clone: polyclonal
form: buffered
aqueous solution
species reactivity:
mouse
technique(s): direct
ELISA: 1:10,000
application(s): research
pathology
shipped in: dry
ice
storage temp.: −20°C
target post-translational modification: unmodified
General description
Immunoglobulin G (IgG) is a glycoprotein antibody that
regulates immune responses such as phagocytosis and is also involved in the
development of autoimmune diseases. Mouse IgGs have four distinct isotypes,
namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in
mice. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for mouse
IgGs.
Immunogen
Purified mouse IgG
Application
Anti-Mouse IgG (whole molecule)-Peroxidase antibody is
suitable for use in immunoblot (1:2000). The product can also be used for
direct ELISA: 1:10,000.
Western blot analysis of transfected Hela cell lysates was
performed using HRP conjugated goat anti-mouse IgG as the secondary at a
dilution of 1:4000.[1]
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4
containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson,
M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques,
Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Other Notes
2024
CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
