biological source:
rabbit
Quality Level: 200
Conjugate: peroxidase
conjugate
antibody form: IgG
fraction of antiserum
antibody product type:
secondary antibodies
clone: polyclonal
form: buffered
aqueous solution
technique(s):
direct ELISA: 1:30,000
dot blot: 1:2,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot (chemiluminescent): 1:160,000
shipped in: dry
ice
storage temp.: −20°C
target post-translational modification: unmodified
General description
Immunoglobulin is a glycoprotein, with two heavy chain and
two light chain connected by disulfide bond. Immunoglobulin g (IgG) is a major
class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. Rabbit
anti-Goat IgG is then conjugated to peroxidase by protein cross linking with
0.2% glutaraldehyde. The conjugate is provided as a solution in 0.01 M
phosphate buffered saline, pH 7.4, containing 0.05% MIT as a preservative.
Immunogen
Purified goat IgG
Application
Anti-Goat IgG (whole molecule)-Peroxidase antibody produced
in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Immunoglobulin mainly helps in immune defense.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III
reaction.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4,
containing 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by
Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7,
7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
