Immunogen
Highly pure (>98%) recombinant rat IL-1beta.
Application
Anti-Interleukin-1β Antibody is an antibody against
Interleukin-1β for use in ELISA, WB, NEUT.
ELISA: 0.5 μg/mL this dilution will allow detection (in
direct ELISA) of
0.2-0.4 ng/well when rat IL-1(is used to coat the plate.
Western blot: 0.1-0.2 μg/mL, this dilution should offer detection limit of
1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Neutralization: to yield ½ maximal inhibition (ND50) of the biological activity
of rat IL-1beta (0.2 ng/mL), a concentration of 0.09-0.14 μg/mL of antibody is
required.
Final working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Biochem/physiol Actions
Reacts with rat Interleukin-1beta (IL-1beta).
Physical form
Affinity purified Immunoglobulin. Lyophilized from 0.5X PBS
pH 7.4, no preservatives. Reconstitute in sterile distilled water.
Preparation Note
Maintain lyophilized material desiccated below 0°C. After
reconstitution maintain at -20°C in convenient undiluted aliquots for up to 6
months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis
for the lot-specific concentration.
Replaces: 04-462; MABF22
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt,
Germany
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
