Product Name: Anti-GAPDH
Antibody, from rabbit, purified by affinity chromatography
biological source:
rabbit
Quality Level: 100
antibody form: affinity
isolated antibody
antibody product type:
primary antibodies
clone: polyclonal
purified by: affinity
chromatography
species reactivity:
mouse, human, rat
species reactivity (predicted by homology):
guinea pig (based on 100% sequence homology), bovine (based
on 100% sequence homology)
technique(s): western
blot: suitable
NCBI accession no.:
NP_002037
UniProt accession no.:
P04406
shipped in: wet
ice
target post-translational modification: unmodified
Gene Information:
human ... GAPDH(2597)
mouse ... Gapdh(14433)
General description
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an
ubiquitous glycolytic enzyme present in moderately high levels in almost all
tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of
1,3-biphosphoglycerate, a "high energy" intermediate used for the
synthesis of ATP. Besides its cytoplasmic action in metabolism it is also
involved in the initial stages of apoptosis or oxidative stress response where
GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH
in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also
been found to bind specifically to proteins implicated in the pathogenesis of a
variety of neurodegenerative disorders including the beta-amyloid precursor
protein and the huntingtin protein where decreased function of GAPDH is
associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has
also been identified as a potential target for nitric oxide (NO)-mediated
cellular toxicity. The complete and functional enzyme is a tetramer with each
of four identical subunits occupying the vertex of a tetrahedron. Binding
domains also include one principally interacting with NAD+ and another
interacting with glyceraldehyde 3-phosphate (GAP).
~36kDa observed
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the
C-terminus of human GAPDH.
Application
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is
popular affinity purified polyclonal antibody. This antibody is supported by
peer reviewed publications and reliably detects Glyceraldehyde-3-Phosphate
Dehydrogenase (GAPDH) is validated for use in WB.
Research Category
Signaling
Biochem/physiol Actions
This antibody recognizes GAPDH at the C-terminus.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M
Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected GAPDH on 10 µg of
HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis
for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
