General description
CyDyes are a family of fluorophores that can be used for
labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are
small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution
and have tolerance to DMSO. They are more photo-stable than fluorescein, have
high molar extinction coefficients, and favorable quantum yields. Due to their
physical and chemical properties, they are used (mainly Cy3 and Cy5) in many
different biological assays such as: DNA microarrays, protein microarrays,
two-dimensional protein analysis (2D gels), fluorescence resonance energy
transfer (FRET), and immunocytochemistry.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody recognizes
Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
Immunogen
mixture of proteins labeled with Cy5.
Application
Anti-Cy5 antibody, Mouse monoclonal has been used in
immunofluorescence assays and western blotting.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody is used to
tag CyDye 5 for detection and quantitation and signal applification by or
within immunocytochemical and immunohistochemical (IHC) techniques such as
ELISA, immunoprecipitation, immunocytochemistry, dot blot assay, and protein
microarrays. Anti-Cy5 antibody is an important tool for Cy5 signal
amplification in assays where biomolecules are labeled with Cy5.
Biochem/physiol Actions
Monoclonal Anti-Cy5 recognizes Cy5 and AlexaFluor647 labeled
proteins, but not Cy3 labeled proteins.
The antibody recognizes Cy5 and AlexaFluor 647 labeled
proteins, but not Cy3 labeled proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4,
containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
