biological source:
goat
Quality Level: 300
Conjugate: unconjugated
antibody form: affinity
isolated antibody
antibody product type:
secondary antibodies
clone: polyclonal
form: buffered
aqueous solution
technique(s):
indirect ELISA: 1:50,000
quantitative precipitin assay: 2.0 mg/mL
shipped in: dry
ice
storage temp.: −20°C
target post-translational modification: unmodified
General description
Anti-Human IgG (Fab specific) antiserum is produced in goat
using purified human IgG Fab fragment as the immunogen. Affinity isolated
antibody is obtained from goat anti-human IgG antiserum by immunospecific
purification which removes essentially all goat serum proteins, including
immunoglobulins, which do not specifically bind to the Fab fragment of human
IgG. IgG is a larger molecule with a molecular weight of 150 kilo daltons
(kDa). IgG has two light chains (L) and two heavy chains (H). The light chain can
be either lambda (λ) or kappa (κ) chain. Functionally, immunoglobulins have
constant region and variable region. Light chain has one variable region in the
N-terminal VL and followed by one constant domain CL. The light chain is
approximately about 25 kDa. The heavy chain has one variable region in the
N-terminal and followed by three constant domains. In between the first (C1H)
and second constant (C2H) domain in the spacer hinge region which connects both
the heavy chains. Each heavy chain is about 55 kDa.
Immunogen
Purified human IgG Fab fragment.
Application
Anti-Human IgG (Fab specific) antibody produced in goat is
effective as a second antibody reagent in immunoassay procedures. It may be
used:
- as
starting material for conjugates using enzymes or fluorescent dyes
- in
enzyme linked immunosorbent assay
- as a
cross-linking antibody in internalisation of 125I-IgG by
streptolysin-O-permeabilised cells
- as
capture antibody in enzyme-linked immunosorbent assay (ELISA) for
detection and semiquantitation of Fab
- in
electrophoresis and immunoelectrophoresis
Biochem/physiol Actions
Enzymatic cleavage of IgG yields antigen-binding (Fab) and
effector activating (Fc) fragments. IgG treatment with papain produces two Fab
fragments and one Fc fragment. IgG treatment with pepsin produces (Fab′)2 fragment
alone. IgG antibodies regulate several functions such as complement activation
and phagocytosis. Thus they play a crucial role in facilitating cytological
immune responses. Anti-human IgG (Fab specific) antibody can be used in
Ouchterlony double diffusion. Due to lack of interspecies cross reactivity to
mouse or rat ascites fluids, this antibody is also ideal for screening human
monoclonal antibodies produced by hybridoma cells grown in vivo.
Goat anti-human IgG (Fab specific) antibody reacts
specifically with Fab fragment of human IgG and shows no reactivity for Fc
fragment of human IgG. This product has no interspecies cross reactivity for
mouse and rat IgG.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4,
containing 15 mM sodium azide as preservative
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
