biological source:
mouse
Quality Level: 100
antibody form: purified
immunoglobulin
clone: 9H8.1,
monoclonal
species reactivity (predicted by homology): all
technique(s): western
blot: suitable
isotype: IgG1κ
shipped in: wet
ice
target post-translational modification: unmodified
General description
Dinitrophenol (DNP) is a small water-soluble, hydrophobic
ligand that binds to transthyretin at acidic pH and also reduces the formation
of amyloid fibril. DNP levels of 100 mg/l or more is greatly toxic to
acclimated bacteria. DNP has been associated with possible therapies for
neurodegenerative and neurological disorders.
Various kDa observed. The Oxyblot protein standards (S7151)
were used to test this antibody. These standards are phosphorylase B, BSA, OVA,
Carbonic Anhydrase, and trypsin inhibitor which all contain 1-3 DNP residues
per protein.
Immunogen
KLH-conjugated DNP
Application
This Anti-DNP Antibody, clone 9H8.1 is validated for use in
WB for the detection of DNP.
Biochem/physiol Actions
Demonstrated to react with a DNP moiety in a wider range of
species. This antibody′s reactivity is expected to be species independent.
Physical form
Format: Purified
Analysis Note
Control
Oxyblot protein standards
Evaluated by Western Blot in Oxyblot protein standards.
Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of
Oxyblot protein standards.
