biological source:
rabbit
Quality Level: 200
Conjugate: unconjugated
antibody form: affinity
isolated antibody
antibody product type:
primary antibodies
clone: polyclonal
form: buffered
aqueous solution
mol wt: antigen
~23 kDa
species reactivity:
human
concentration: ~1.0 mg/mL
technique(s):
immunohistochemistry: 20 μg/mL using formalin-fixed,
paraffin-embedded human heart
indirect immunofluorescence: 1-2 μg/mL using MCF7 cells
western blot: 1-2 μg/mL using extracts of OVCAR-3 cells
UniProt accession no.:
O00501
shipped in: dry
ice
storage temp.: −20°C
target post-translational modification: unmodified
Gene Information: human
... CLDN5(7122)
General description
Claudin-5 (CLDN5) is encoded by the gene mapped to human
chromosome 22q11.21. The encoded protein a 23kDa, four-transmembrane protein.
It is mainly expressed in endothelial cells forming part of the blood-brain
barrier (BBB).
Immunogen
synthetic peptide corresponding to a sequence at the
C-terminus of human claudin-5, conjugated to KLH. The corresponding sequence is
highly conserved in mouse claudin-5 (single amino acid substitution) and in rat
claudin-5 (89% identity).
Application
Anti-Claudin-5 (C-terminal) antibody has been used in I
- immunoblotting
- immunofluorescence
- immunohistochemistry
- immunolabelling
- immunocytochemistry
Biochem/physiol Actions
Claudin-5 (CLDN5), as an essential component of blood-brain
barrier (BBB), plays a vital role in regulating the homeostasis of the central
nervous system. Mutation in the gene increases the risk of susceptibility to
schizophrenia. Overexpression of the gene is associated with the progression of
pancreatic ductal adenocarcinomas. Loss of CLDN5 in cardiomyocytes and
endothelial cells is one of the main reason behind human heart failure. Hence,
early usage of CLDN5 can be considered as a potential therapeutic target for
heart failure.
Claudin-5 has been shown to be overexpressed in various
tumors including lung adenocarcinomas but undetectable in squamous cells cell
carcinomas.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4,
containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company
documentation accompanying the product(s), our products are intended for
research use only and are not to be used for any other purpose, which includes
but is not limited to, unauthorized commercial uses, in vitro diagnostic uses,
ex vivo or in vivo therapeutic uses or any type of consumption or application
to humans or animals.
