General description
Free radicals or reactive oxygen species (ROS) are produced
during biochemical redox reactions as part of normal physiological cell
metabolism (protection from infectious organisms) and as a response to
environmental factors such as UV light, cigarette smoke, environmental
pollutants, and g-radiation. Once formed, ROS attack cellular components
causing damage to lipids, proteins, and DNA, which can initiate numerous
diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes,
liver damage, and central nervous system disorders.
In the presence of antioxidants the radical cation is
suppressed to an extent dependent on the activity of the antioxidant and the
color intensity is decreased proportionally. Trolox, a water-soluble vitamin E
analogue, serves as a standard or a control antioxidant.
Application
Antioxidant assay kit provides all of the reagents required
for an efficient measurement of the total antioxidant capacity of plasma,
serum, urine, saliva, cells, and tissue lysates.[1] It has also been used
to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline
sulphonate]) (ABTS+) scavenging capacity.[2][3]
It was tested on A431 and CHO cell lysates; rat brain,
liver, and kidney lysates; human plasma, serum, urine, and saliva.
Suitable for the measurement of the total antioxidant
capacity of plasma, serum, urine, saliva, cells, and tissue lysates
Biochem/physiol Actions
The principle of the antioxidant assay is formation of a
ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which
oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to
produce a radical cation, ABTS·+ , a soluble chromogen that is green in color
and can be determined spectrophotometrically at 405 nm.
Preparation Note
Myoglobin Solutions are to be stored at -20 °C after
reconstitution.
Trolox Working Solution is to be stored at -20 °C after reconstitution.
ABTS Substrate Solution is to be stored at 4 °C for 2 weeks or at -20 °C for 1
month.
Reagents are to be prepared with ultrapure water.
Legal Information
