The Alkaline Phosphatase (ALP) Activity Kit is a highly
reliable and sensitive assay designed for the quantitative measurement of ALP
activity in various sample types. ALP is a group of cytomembrane-related
enzymes with hydrolysis and transfer activity, acting on a variety of phosphate
substrates. ALP is a homologous dimerase where each catalytic site contains
three metal ions.
The assay is based on the ability of alkaline phosphatase to decompose benzene
disodium phosphate to produce free phenol and phosphoric acid. Phenol reacts
with 4-aminopyrine in alkaline solution and oxidizes with potassium
ferricyanide to form a red quinone derivative. The enzyme activity can be
calculated indirectly by measuring the OD value.
Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and
providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important
metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and
reliable results
Assay Range0.13–50 King Units/100 mL
Assay Sensitivity0.13 King Units/100 mL
For Use With (Equipment)Microplate Reader
Incubation Time15 min.
Interassay CV8.5
Intraassay CV5.1
Quantity96 tests
Sample Volume5 μL
Shipping ConditionDry Ice, 2–8°C
Target Kit NamedAlkaline Phosphatase (ALP)
Total Assay Time30 min.
Detection MethodColorimetric
Sample TypePlasma, Serum, Tissue Homogenates, Whole
Cells
Unit SizeEach
