General description
An antioxidant is a molecule capable of slowing or
preventing the oxidation of other molecules. Antioxidants protect the cells
from damages by reactive oxygen species which are produced in oxidation
reactions in the cell. Antioxidants can be small molecules such as glutathione,
vitamins, or macromolecules such as catalase, glutathione peroxidase. As
oxidative stress contributes to the development of many diseases including
Alzheimer′s disease, Parkinson′s disease, diabetes, rheumatoid arthritis and
neurodegeneration, the use of antioxidants in pharmacology is intensively
studied. Antioxidants are also widely used as dietary supplements and in
industry as preservatives in food, cosmetics, rubber and gasoline. Simple,
direct and high-throughput assays for total antioxidant capacity (TAC) find
wide applications in research, food industry and drug discovery.
Application
The Antioxidant Assay Kit may be used for:
- Cancer
Research
- Diabetes
Research
- Drug
Discovery
- Neurodegenerative
Disease Research
- Oxidative
Stress Research
This kit is suitable for antioxidant determination in serum,
plasma, urine, saliva and other biological samples, food and beverages. The kit
can also be utilized in drug discovery/pharmacology for studying the effects of
drugs on TAC.
Biochem/physiol Actions
The Antioxidant Assay Kit′s improved assay measures total
antioxidant capacity in which Cu2+ is reduced by antioxidant to
Cu+. The resulting Cu+ specifically forms a colored
complex with a dye reagent. The color intensity at 570nm is proportional to TAC
in the sample. The kit uses 20 μL of sample and has a linear detection range
from 1.5 to 1000 μM Trolox equivalents.
Features and Benefits
Broad Detection Range: Detect antioxidants
accurately across a wide range, from 1.5 µM to 1000 µM Trolox equivalents,
using only 20 μL of sample in a 96-well plate setup, ensuring comprehensive
analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the
addition of only a single working reagent and a 10 minute room temperature
reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our
kit into high-throughput handling systems, ensuring smooth and accurate
processing, enhancing efficiency in your laboratory workflow.
Compatible with high-throughput handling systems.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
