Quality Level: 100
application(s): pharmaceutical
detection method: fluorometric
storage temp.: −20°C
General description
Kinases, alternatively referred to as phosphotransferases,
form an extensive family of enzymes responsible for transferring phosphate
groups from the high-energy donor molecule ATP to their designated substrates.
This family of enzymes is renowned for its role in regulating a myriad of
cellular processes, with the protein kinases constituting the largest subgroup.
In humans, 518 distinct kinases have been identified, and a substantial portion
of human proteins, up to 30%, undergo modification by these kinases. The
considerable diversity and pivotal role of kinases in cellular signaling
position them as promising targets for drug development, biochemical and
biomedical research.
Application
The Kinase Assay Kit may be used for:
- Cancer
Research
- Cell
Signaling Research
- Drug
Discovery
The kit is suitable for the determination of protein kinase
activity and for high-throughput screening of kinase inhibitors.
Biochem/physiol Actions
The Kinase Assay Kit provides a simple and rapid method for
assaying kinase activity and high-throughput screening for kinase inhibitors.
This homogeneous microplate-based assay involves incubating the kinase with a
single working reagent, in which ADP is enzymatically converted to ATP and
pyruvate, which is quantified using a fluorometric (Ex = λ530 nm/λEm = 590 nm)
assay method.
Features and Benefits
- High
Sensitivity and Accuracy: Achieve quantification of kinase activity,
detecting as low as 0.01 U/L with high sensitivity and accuracy.
- Streamlined
Procedure: Effortlessly conduct the assay with a simplified process,
adding just a single working reagent. Complete the assay in a quick
10-minute reaction, optimizing efficiency and saving valuable time.
- High-Throughput
Inhibitor Screening: Screen for kinase inhibitors effectively with a
minimal 5 μL test compound requirement, enhancing efficiency in
high-throughput settings.
Other Notes
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