uality Level: 100
application(s): pharmaceutical
detection method: turbidimetric
relevant disease(s):
cancer
storage temp.: −20°C
General description
Hyaluronidases are a family of enzymes that catalyze the
degradation of the glycosaminoglycan, hyaluronic acid. Hyaluronic acid is one
of the major constituents of the extracellular matrix in organisms where it
contributes to both cell proliferation and migration. The role of
hyaluronidases in breaking down this key factor in cell proliferation makes
them a possible target for cancer treatment. One hypothesis is that increased
hyaluronidase may help prevent tumor invasion by breaking down the extracellular
matrix needed for tumor expansion. Conversely, decreasing hyaluronidase
activity might prevent metastasis by stopping cancer cells from escaping
primary tumor masses. The study to determine hyaluronidases′ exact role in
cancer pathology is still ongoing.
Application
The Hyaluronidase Inhibitor Screening Assay Kit may be used
for:
- Cancer
Research
- Drug
Discovery
The kit is suitable for the high-throughput screening for
evaluation of hyaluronidase inhibitors.
Biochem/physiol Actions
The Hyaluronidase Inhibitor Screening Assay Kit uses a two
step turbidimetric reaction to measure hyaluronidase activity by the amount of
hyaluronic acid that is hydrolyzed. A stop reagent halts the enzymatic reaction
and forms turbidity with any residual hyaluronic acid in the well. The decrease
in turbidity at A600 nm is directly proportional to the hyaluronidase activity
in the sample.
Features and Benefits
Efficient Inhibitor Screening: Screen for
Hyaluronidase inhibitors using only 20 μL of test compound with a quick 30
minute protocol, offering a streamlined and effortless procedure.
Compatibility with High-Throughput Systems: Easily incorporate our
kit into high-throughput handling systems, ensuring smooth and accurate
processing, enhancing efficiency in your laboratory workflow.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
