General description
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor,
Endotoxin-Free is designed for use in cell culture systems and animal perfusion
studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin
family,[1] contains three domains and two sub-domains under each. It is a
non-glycosylated globular, α-helical protein that has 17-disulfide bonds.[2][1]
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor,
Endotoxin-Free has been used in the preparation of advanced glycation
end-products (AGEs).[3] It has also been used to dilute lipopolysaccharide
(LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of
whole blood cells.[4]
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the most important constituent
of cell culture media. It aids embryonic stem cells (hESC)
differentiation.[5] BSA facilitates the transport of drugs, hormones, and
fatty acids.[2] It elicits high solubility and is suitable in the cosmetic
and pharmaceutical industries.[6] It is applicable as a blocking agent in
enzyme-linked immunosorbent assay (ELISA).[7]
Preparation Note
Following reconstitution, store in the refrigerator (4°C).
0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to
3 months at 4°C.
Material may take up to 24 h to fully dissolve.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA,
Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
