General description
PRONASE® Protease, Streptomyces
griseus, nuclease-free is a non-specific protease that liquefies mucins and
digests proteins to free amino acids. This mixture can be used to isolate
living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of
pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and
produces more efficient dissociation than collagenase.[1]
Application
Pronase® Protease, Streptomyces
griseus, nuclease-free has been used:
- in
single-cell preparations for cardiac side population (CSP) analysis[1]
- in
aflatoxin plasma biomarker assay[2]
- to
permeabilize Caenorhabditis elegans embryo for in
vivo micrococcal nuclease digestion[3]
Biochem/physiol Actions
Proteases catalyze the hydrolysis of proteins to form
peptides and free amino acids. They also regulate the breakdown of fats and
carbohydrates through many enzymatic cascades and activating proenzyme
zymogens.[4]
Preparation Note
Unstable in solution; reconstitute just prior to use.
Other Notes
Note: 1 KU = 1000 units.
One unit is defined as the amount of enzyme that will
liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C,
pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA,
Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt,
Germany
Disclaimer
Toxicity: Harmful (C)
