The Thermo Scientific Pierce Hanks' Balanced Salt Solution
(HBSS) without Ca2+, Mg2+ is ideal for gently washing primary cells after
digestion of the connective tissue. The Thermo Scientific Pierce Primary
Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the
optimal yield and viability of primary cardiomyocytes from neonatal heart from
mouse and rat.
Features of the Thermo Scientific Pierce Hanks' Balanced Salt Solution
(HBSS):
• Optimized – procedure and reagents optimized for viability,
yield, purity, and ease-of-use
• Time-saving – isolation protocol requires less than 2 hours
compared to up to 21 hours by other methods
• Yield – provides a 1.5- to 4-fold increase in yield compared
to do-it-yourself methods and other commercial kits
• Viability – provides higher viability than do-it-yourself
methods and other commercial kits
• Functional – cultured cardiomyocytes express the appropriate
biochemical markers with validated beating activity
Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte
Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell
Isolation, and Cardiomyocyte Growth Supplement
Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive
disease modeling
The Primary Cardiomyocyte Isolation Kit has been designed to obtain high
viability and purity of primary cardiomyocytes using validated reagents and an
optimized procedure for enzymatic digestion and culturing. The kit contains two
tissue-specific dissociation enzymes, media formulated for primary cell
culture, and a cardiomyocyte cell culture growth supplement that promotes the
isolation, enrichment and growth of functional cardiomyocytes. The validated
protocol ensures optimal isolation and significantly reduces the processing
time of isolation when compared to other methods. Addition of the cardiomyocyte
growth supplement to culture cells further enriches for cardiomycytes, and
cultures have been maintained for up to 7 days using the kit components.
Current methods for dissociation of cardiac cells from heart tissue require
repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion,
resulting in reduced yield and viability of isolated cells. The Pierce Primary
Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol
that prevents overdigestion for the isolation and culture of cardiomyocytes.
The complete process from handling primary tissues to seeding cells in culture
vessels can be completed within 2 hours.
Because it uses optimized tissue-specific dissociation enzymes, this kit isolates
cardiomyocytes with greater yield and cell viability than cells isolated by
existing methods. The isolated primary cardiomyocytes express cardiomyocyte
protein markers and exhibit contractile function (beating activity, see video),
thereby providing a model system for studies of contraction, ischaemia, hypoxia
and the toxicity of various compounds.
