Application
C2C12 Cell Line has been used to study the the expression of
OXT and OXTR (oxytocin and its receptor) genes and proteins[1] and in
differentiation studies.[2]
Biochem/physiol Actions
Mouse C3H muscle myoblast
Subclone from myoblast line established from normal adult
C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting
myotubes expressing characteristic muscle proteins. Provides model to
study in vitro myogenesis and cell differentiation.
Preparation Note
DMEM +
2mM Glutamine +
10-15% Foetal Bovine Serum FBS / FCS.
Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at
1-2x1,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA;
5% CO2; 37°C. Do not allow cells to reach confluence.
Other Notes
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