General description
Cell freezing medium is a combination of cryoprotectant DMSO
with fetal bovine serum. However serum free medium replaces serum with
methylcellulose and poloxamer 188 and other additives like ascorbic acid,
α-tocopherol and proline.[1]
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the
freezing of human neural progenitor cells (hNPCs),[2] olfactory
ensheathing cells (OECs)[3] and retinal pigment epithelial (RPE) cells.[4]
Other Notes
Complete ready-to-use medium containing Minimum Essential
Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to
the formulation of Waymouth[5].
