General description
This DMEM-Hi glucose medium is a 1x complete medium with
sodium pyruvate added. It also differs from the original DMEM-Hi formulation
wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable
component of media.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification
of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino
acids and vitamins. The original formulation contained 1000 mg/L of glucose and
was used to culture embryonic mouse cells. Since then, it has been modified in
several ways to support primary cultures of mouse and chicken cells, as well as
a variety of normal and transformed cells. Each of these media offers a
different combination of L-glutamine and sodium pyruvate. Additionally, the
glucose levels have been raised to 4500 mg/L, contributing to the name
"DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been
used for cell culture.[1][2][3]
