General description
Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced
salt solution (BSS) used for the handling and culturing of mammalian cells.
DPBS buffer is used to irrigate, wash, and dilute mammalian cells while
maintaining osmotic balance. It provides water and inorganic ions essential for
normal cell metabolism. DPBS with calcium and magnesium facilitates cell
binding and clumping. Dulbecco′s PBS without these ions can be used to wash and
rinse suspended cells. Phosphate buffering maintains the pH in the physiological
range.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
- for
the dilution of fresh red blood cells to study the trapping of red blood
cell aggregate.[1]
- to
wash the wells of a microtiter plate.[2]
- to
wash the cells before resuspending them in lysis buffer for DNA
isolation.[3]
- as a
component of embryo holding medium for vitrification of embryos.
Other Notes
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