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Sigma-Aldrich® HEPES solution, 1 M, suitable for cell culture

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CAS Number:  7365-45-9

MDL number:  MFCD00006158

UNSPSC Code:  12161700

PubChem Substance ID:  329815199

NACRES:  NA.25

Product Name:  HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Grade:  BioPerformance Certified

Quality Level:  200

Sterility:  0.2 μm filtered

Form:  liquid

Concentration:  1 M

technique(s):  cell culture | mammalian: suitable

impurities:

Bioburden, tested

DNase, RNase, Protease, Nickase, free

endotoxin, tested

pH:  5.0-6.0

useful pH range:  6.8-8.2

cation traces:

Fe: <5 ppm

heavy metals (as Pb): <5 ppm

Suitability:  suitable for molecular biology

application(s):  diagnostic assay manufacturing

SMILES string:  OCCN1CCN(CCS(=O)(O)=O)CC1

InChI:  1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key:  JKMHFZQWWAIEOD-UHFFFAOYSA-N

 




General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Application

RNAse HEPES has been used:


  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets[3]
  • To recover purified ribonucleotide[4]
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing[5]
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets[6]