-
  1. All Products
  2. Cell Culture & Cell Biology
  3. Sigma-Aldrich® Kanamycin disulfate salt from Streptomyces kanamyceticus aminoglycoside antibiotic

Sigma-Aldrich® Kanamycin disulfate salt from Streptomyces kanamyceticus aminoglycoside antibiotic

https://mtmedicalscience.com/web/image/product.template/2259/image_1920?unique=4600b47
(0 review)

MT-CC-K1876-1G

สั่งซื้อสินค้า / Order Now 

**สั่งซื้อสินค้าคลิกที่ปุ่ม Buy Now***

➙Buy Now


*กรณีแสดงราคา 0 บาท กรุณาติดต่อบริษัทเพื่อสอบถาม ราคาขาย*

0.00 ฿ 0.0 THB 0.00 ฿

0.00 ฿

Not Available For Sale

This combination does not exist.

Contact Us

CAS Number:  64013-70-3

MDL number:  MFCD00143916

UNSPSC Code:  51281654

PubChem Substance ID:  329817127

NACRES:  NA.76

biological source:  Streptomyces kanamyceticus

Quality Level:  200

Form:  powder

Potency:  ~650 μg per mg

Impurities:  ≤4% Kanamycin B

Color:  white to off-white

Solubility:  H2O: 10 mg/mL

antibiotic activity spectrum:

Gram-negative bacteria

Gram-positive bacteria

mycobacteria

mycoplasma

Mode of action:  protein synthesis | interferes

SMILES string:

OS(O)(=O)=O.OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI:

1S/C18H36N4O11.2H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;2*1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;2*(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;;/m1../s1

InChI key:  OGTKIXVMLDAMNU-KNQICTBBSA-N





General description

Chemical structure: aminoglycoside

Application

Kanamycin sulfate is a broad spectrum minoglycoside-antibiotic It is used as an additive in culture media for the isolation of group D Streptococci on Kanamycin Esculin Azide Agar (Mossel et al.) or for selection of transformed plant cells containing the neomycin phosphotransferase (for kanamycin resistance); on a kanamycin-medium[1][2]. Kanamycin has been used for electrophysiological recordings of outer hair cells and inner hair cells[3]. It is used to inhibit shoot regeneration from Siberian Elmleaf explants[4]. It is recommended for use in cell culture applications at 100 mg/mL.

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial Spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Preparation Note

Kanamycin acid sulfate is prepared by solubilizing kanamycin sulfate in dilute sulfuric acid and drying. The sulfate content is approximately 25%.

Kanamycin sulfate is soluble in water at 10 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Disclaimer

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.