General description
Eagle′s minimum essential medium (MEM) is a synthetic
culture medium, which is composed of balanced salt solutions, and amino acids
and vitamins. These components are essential for the growth of cultured cells.
MEM might be supplemented with non-essential amino acids, to make MEM
non-essential amino acid solution. The non-essential amino acids include
L-alanine, L-asparagine, L-aspartic acid, L-glycine, L-serine, L-proline and
L-glutamic acid.[1]
Application
Adding supplements of amino acids to media both stimulates
growth and prolongs the viability of cells in culture. MEM Non-essential Amino
Acid Solution supplement adds nutrients and reduces the biosynthetic burden on
cell in vitro.
MEM Non-essential Amino Acid Solution has been used in Dulbecco′s minimum
essential media for the maintenance of mammalian cells.[2] It has been
supplemented in medium prepared for oocyte maturation.[3][4]
Biochem/physiol Actions
Studies on hybridoma culture shows that addition of Eagle′s
minimum essential medium (MEM) non-essential amino acid leads to enhanced
membrane stability, during death phase of the culture. This also results in
increased antibody secretion during death phase, and decreased lactate
concentration.[5]
Other Notes
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