General description
JumpStart™ REDTaq® ReadyMix™PCR
Reaction Mix is a prepared solution combining the performance benefits of hot
start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master
mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides,
buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL
of the ReadyMix to primers, template, and water to a final reaction volume of
50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA
polymerase at room temperature. During the first denaturation step of PCR the
complex dissociates and polymerase activity is fully restored. The inert red
dye does not effect automated sequencing, restriction enzyme digestion,
ligation, or other downstream manipulations. The PCR product is easily
separated from the dye by standard purification methods if desired. The hot
start mechanism allows for room temperature set up, making it the ideal for
high throughput applications.
Application
Jumpstart™ REDTaq® ReadyMix™ Reaction
Mix is used for direct DNA extraction and amplification using
PCR.[1][2][3][4] It has also been used for:
- qualitative
multiplex PCR[1]
- methylation-specific
PCR (MSPCR)[5]
- PCR
amplification of cDNA synthesized from total RNA[6]
Features and Benefits
- JumpStart™ REDTaq® DNA
polymerase, an antibody inactivated hot start enzyme, is designed to
minimize non-specific amplification while increasing target yield &
specificity
- JumpStart
Taq DNA polymerase provides superior amplification regardless of template
concentration
- REDTaq
JumpStart ReadyMix reduces pipetting steps and risk of contamination. The
hot start mechanism allows for room temperature set up, making it the
ideal for high throughput applications
- Inert
red dye allows for easy verification that reagent has been mixed properly
- Other
loading dyes are not required. Aliquots may be directly loaded onto an
agarose gel after the reaction
Packaging
Default reaction volume is 50 μL
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid
precipitable DNA in 30 min. at 74 °C.
Other Notes
For a typical PCR reaction, mix 25 μL of JumpStart™ REDTaq® ReadyMix™ with
25 μL of a mixture containing template DNA, primers, and water. Reaction volume
can be scaled if desired.
View more detailed information on JumpStart REDTaq and
ReadyMix products at www.sigma-aldrich.com/hotstart.
Legal Information
Purchase of this product includes an immunity from suit
under patents specified in the product insert to use only the amount purchased
for the purchaser′s own internal research. No other patent rights (such as 5′
Nuclease Process patent rights) are conveyed expressly, by implication, or by
estoppel. Further information on purchasing licenses may be obtained by
contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre
Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent
No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the
following US patents and corresponding patent claims outside the US: US
8,404,464 and US 7,972,828. The purchase of this product includes a limited,
non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt,
Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
