General description
Collagenase type I (from Clostridium histolyticum) is a
crude collagenase preparation that can be used for the isolation of primary
cells or for tissue dissociation by enzymatic means. The preparation also may
contain caseinase, clostripain, and tryptic activities. This preparation can be
used to dissociate cells in culture, and has been successfully used to passage
human embryonic stem cells cultured with Millipore’s HEScGROTM Animal
Component-Free Medium.
Packaging
1 vial containing 250mg of lyophilized powder
Physical form
Collagenase type I is supplied as a dialyzed, lyophilized
powder.
Preparation Note
Collagenase type I should be stored in its powdered form at
2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and
stored at -20oC, but should not be repeatedly thawed and re-frozen.
Analysis Note
Each lot of collagenase type I is assayed via a modification
of Mandl (1964), with the extent of collagen breakdown determined using the
colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then
tested for successful passage of human embryonic stem cells cultured in
Millipore′s HEScGRO Medium for hES cells. Cells are characterized for correct
marker expression and morphology after five passages.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt,
Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when
intended to be used for scientific purposes, including for import and export
activities (Article L 1211-1 paragraph 2 of the Public Health Code). The
purchaser (i.e. enduser) is required to obtain an import authorization from the
France Ministry of Research referred in the Article L1245-5-1 II. of Public
Health Code. By ordering this product, you are confirming that you have
obtained the proper import authorization.
Safety Information
