Empirical Formula (Hill Notation): C27H32N6·Cl2
CAS Number: 91416-20-5
Molecular Weight: 511.49
UNSPSC Code: 12352125
UNSPSC Code: 12352200
NACRES: NA.77
Quality Level: 100
Assay: ≥98%
(HPLC)
Form: powder
Color: red to
black
Solubility: DMSO:
2 mg/mL, clear
storage temp.: -10
to -25°C
SMILES string:
[N-]=[N+]=NC1=CC2=C(C=C1)C3=C(C=C(C=C3)N)[N+](CCC[N+](CC)(C)CC)=C2C4=CC=CC=C4.[Cl-].[Cl-]
InChI key: NUMTTYQOTJKZSE-UHFFFAOYSA-M
Biochem/physiol Actions
Propidium derivative that allows light-induced crosslinkage
to DNA for highly selective live-dead cells analysis by PCR or fluorescence
microscopy.
Propidium monoazide (PMA) is a propidium derivative with an
azide group that allows crosslinkage to DNA upon light exposure. PMA is only
permeable to bacteria with compromised membrane integrity, making it highly
selective for sick/dying/dead cells over healthy cells. PMA is typically added
to cell samples (50 μM for 1-15 min in the dark) prior to cross-linking
induction (650-W halogen light source), washing and DNA extraction. Due to its
higher charge, PMA (+2) is less permeant to live cells and more selective than
ethidium monoazide/EMA (+1) for dead cells. The stained cells can also be
viewed and analyzed by fluorescence microscopy.
