General description
The apparent molecular weight is related to the tagged
protein.
Immunogen
N-terminal His-tagged and truncated TRAP protein
corresponding to amino acids 26-248 from P. falciparum expressed in E coli.
Application
This Anti-Histidine Tagged Antibody (anti His antibody)
clone 4D11 is validated for use in IC, WB for the detection of Histidine
Tagged.
Biochem/physiol Actions
Recognizes tag sequence HHHHHHGS on either the N- or
C-terminus of recombinant proteins; GS amino acids are required for recognition
of 6His tag
Physical form
Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified
immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07%
sodium azide.
Analysis Note
Control
Cells expressing the His-tag
routinely evaluated by immunoblot on recombinant N-terminal
and C-terminal His-tagged proteins
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt,
Germany
