General description
Buffered Peptone Water is rich in nutrients and produces
high resuscitation rates for sub lethally injured bacteria and intense growth.
The phosphate buffer system prevents bacterial damage caused by changes in the
pH of the medium. Peptone including enzymatic digest of casein acts as a source
of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the
osmotic balance.
GranuCult® is our new brand name for our existing granulated
dehydrated culture media which apply to new EN ISO 11133:2014 and various other
ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water
samples. All these media are currently being updated to be fully compliant to
the latest version of these standards and regulations with respect to
composition and quality control and will be available shortly.
Application
Buffered Peptone Water is used in the testing of food and
water samples. This product is suitable for the preliminary non selective
enrichment of bacteria, particularly pathogenic Enterobacteriaceae such
as Salmonella and Cronobacter, from food,
animal feed, water, and other materials. The product is also suitable as a
diluent for general use.
Preparation Note
- Store
at +15 °C to +25 °C, dry and tightly closed.
- Do
not use clumped or discolored medium.
- Protect
from UV light (including sunlight).
- For in
vitro use only.
- According
to EN ISO/FDIS 6579-1, the self-prepared medium can be stored in closed
containers at +2 °C to +8 °C in the dark and protected against evaporation
for up to six months.
Analysis Note
Appearance (clearness): clear
Appearance (colour): yellowish
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO
11133 and the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
≤ 100
Inoculum on reference medium (Salmonella Abony NCTC 6017): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella Abony NCTC 6017): good to very good
Incubation:
18 ± 2 hours at 37 ± 1 °C
S.abony 18 - 24 hours at 30 - 35 °C
Other Notes
Replaces: 1072285007; 1.07228
The prepared medium is clear and yellowish.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA,
Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA,
Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and
the cost-efficient powdered NutriSelect® culture medi.
depending on your needs. The designations basi. plu. or prime are added to
indicate the quality control leve. from basic quality control to standard QC
plus to prime for full regulatory compliance.
