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Sigma-Aldrich® Coomassie Brilliant blue G 250 (C.I. 42655)

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Empirical Formula (Hill Notation):  C47H48N3NaO7S2

CAS Number:  6104-58-1

Molecular Weight:  854.02

MDL number:  MFCD00078482

UNSPSC Code:  41105305

EC Index Number:  228-058-4

NACRES:  NC.01

Quality Level:  100

Potency:  >5000 mg/kg LD50, oral (Rat)

technique(s):  protein staining: suitable

pH:  6.4 (20 °C, 10 g/L in H2O)

solubility:  40 g/L

bulk density:  520 kg/m3

storage temp.:  no temp limit

SMILES string

[Na+].[S](=O)(=O)([O-])c1cc(ccc1)CN(CC)c2cc(c(cc2)[C+](c5c(cc(cc5)N(CC)Cc6cc(ccc6)[S](=O)(=O)[O-])C)c3ccc(cc3)Nc4ccc(cc4)OCC)C

InChI:

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key:  CMGWKYXJIYYESN-UHFFFAOYSA-M

General description

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye.[1] CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[2] CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.[1]

Application

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.[3] It has also been used in Bradford protein assay.[4]

Analysis Note

Identity (UV/VIS-Spectrum): passes test

Absorption maximum λmax. (buffer pH 7.0): 577 - 584 nm

Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570

TLC-Test: passes test

Loss on drying (110 °C): ≤ 8 %

Suitability for electrophoresis: passes test

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