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  2. Sigma-Aldrich® 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ,

Sigma-Aldrich® 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ,

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Empirical Formula (Hill Notation):  C18H24N6O6S4

CAS Number:  30931-67-0

Molecular Weight:  548.68

Beilstein:  7329461

EC Number:  250-396-6

MDL number:   MFCD00010404

UNSPSC Code:   12352204

PubChem Substance ID:   329749485

NACRES:  NA.83

Product Name:  2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)

Quality Level:  200

Assay:  ≥98% (HPLC)

Form:  powder or crystals

Impurities:  ≤6.0% water

Solubility:  H2O: 10 mg/mL, slightly hazy to clear

storage temp.:  2-8°C

SMILES string:   [NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O

InChI:

1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;

InChI key:  OHDRQQURAXLVGJ-AXMZSLBLSA-N

 





Application

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt has been used:


  • in the radical scavenging assay to test the antioxidant property of Pinus densiflora bark (PDB) extracts[1]
  • to test the antioxidant capacity (AOC) of Aloe vera samples[2]
  • as a substrate for streptavidin-horse radish peroxidase (HRPO) in enzyme-linked immunosorbent assay (ELISA)[3]

 

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Other Notes

Chromogenic substrate for peroxidase; The ABTS-H2O2-peroxidase system in EIA.