General description
Most commonly-used phorbol ester. Extremely potent mouse
skin tumor promoter. Activates protein kinase C in vivo and in
vitro, even at nM concentrations. Promotes the expression of inducible NOS
in cultured hepatocytes. Activates Ca2+-ATPase and potentiates
forskolin-induced cAMP formation. Inhibits apoptosis induced by the Fas
antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Its
binding is reversible. Also available as a 10 mM solution in DMSO (Cat.
No. 500582 ).
Biochem/physiol Actions
Cell permeable: no
Primary Target
Protein Kinase C
Product does not compete with ATP.
Reversible: yes
Packaging
Packaged under inert gas
Warning
Toxicity: Harmful (C)
Preparation Note
Further dilute with aqueous buffers just prior to use.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C).
Anhydrous DMSO stock solutions are stable for up to 6 months at -20°C. With
frequent use, the DMSO stock solutions may accumulate water. The presence of
water in the stock solution will accelerate the decomposition of the product,
resulting in significant loss of activity in 2-3 months.
Other Notes
Powell, D.J., et al. 2003. Mol. Cell Biol.23, 7794.
Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8443.
Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia7, 1846.
Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol.31, 1101.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Kontny, E., et al. 1992. Eur. J. Pharmacol.227, 333.
Zanaboni, P.B., et al. 1992. J. Appl. Physiol.73, 2011.
Saltis, J., et al. 1991. J. Biol. Chem.266, 261.
Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA82, 2774.
Perchellet, J. 1985. Carcinogenesis6, 567.
Nishizuka, Y. 1984. Science255, 1365.
Mastro, A. 1982. Lymphokines6, 263.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA,
Darmstadt, Germany
