-
  1. All Products
  2. Chemicals / Reagents
  3. Sigma-Aldrich® Crystal Violet

Sigma-Aldrich® Crystal Violet

https://mtmedicalscience.com/web/image/product.template/2353/image_1920?unique=fbd2fe3
(0 review)

MT-CM-61135-100G


สั่งซื้อสินค้า / Order Now 

**สั่งซื้อสินค้าคลิกที่ปุ่ม Buy Now***

➙Buy Now


*กรณีแสดงราคา 0 บาท กรุณาติดต่อบริษัทเพื่อสอบถาม ราคาขาย*

0.00 ฿ 0.0 THB 0.00 ฿

0.00 ฿

Not Available For Sale

This combination does not exist.

Contact Us

Linear Formula:  C25H30N3Cl

CAS Number:  548-62-9

Molecular Weight:  407.98

Colour Index Number:  42555

Beilstein:   3580948

MDL number:  MFCD00011750

UNSPSC Code:  12171500

PubChem Substance ID:  329759365

NACRES:  MA.02

Form:  powder

Quality Level:  200

technique(s):  microbe id | staining: suitable

color:  green to very dark green

visual transition interval:  0.1-2.0, yellow-green to blue-violet

mp:  205 °C (dec.) (lit.)

density:  1.190 g/cm3

ε (extinction coefficient):  ≥1750 at 585-595  nm in water

suitability:

suitable for microscopy (Bact., Bot., Hist., Vit.)

suitable for microscopy

antibiotic activity spectrum:  fungi

application(s):

diagnostic assay manufacturing

hematology

histology

Mode of action:

cell membrane | interferes

enzyme | inhibits

storage temp.:  room temp

SMILES string:

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI:

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key:  ZXJXZNDDNMQXFV-UHFFFAOYSA-M

General description

Crystal violet, also known as Basic Violet 3 or Gentian Violet, is a basic, cationic aminoarylmethane dye. It is the N-hexamethylated derivative of pararosaniline and bears a lipophilic cation. It is primarily used in bacteriology and microscopy as the primary stain in Gram staining to classify and distinguish between Gram-positive and Gram-negative bacteria. It is indicator grade, exhibiting a color transition from yellow-green in acidic conditions to blue-violet in basic conditions. It also possesses antibacterial and antifungal properties.[1][2]

Application

  • Crystal violet is mainly used in Gram staining and its variants, and for staining amyloid, bacterial components, and vascular plant tissues.
  • It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyze meiotic structures.
  • It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears.[1]

Biochem/physiol Actions

Gram staining is used to differentiate bacteria based on their cell wall structure. Gram-positive bacteria have a thicker cell wall with more peptidoglycan and less lipid content, while gram-negative bacteria have a thinner cell wall with less peptidoglycan and more lipid content. During the staining process, crystal violet is applied to the bacteria, followed by the iodine to form a complex that fixes the dye. A decolorizer is then used to remove the dye from gram-negative bacteria by dissolving their lipid layer. Gram-positive bacteria retain the dye due to the dehydration and shrinking of their cell walls. A counterstain, such as safranin or basic fuchsin, is applied to impart a pink color to decolorized gram-negative bacteria.

The antimicrobial properties of crystal violet are due to free radical generation and the formation of unionized dye-bacteria complexes. It inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.[3]