Application Notes
Zymolyase 20T is used to lyse living yeast cell walls to
produce protoplast or spheroplast of various strains of yeast cells. It may
also be used for glucan hydrolysis. Zymolase-has a low solubility. Use as a
suspension. If it is necessary to make a sterile enzyme solution of more than
.5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer
solution (pH 7.5) which contains 5% glucose. To remove insoluble substances,
filter through a sterile filter. Do not use Nitrocellulose filters. Dilute
sterile enzyme suspension on to desired concentration.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for
research or further manufacturing use only, not for direct human use. For more
information, please contact our customer service department.
Key Applications
Yeast Cell Culture | Used to lyse cell wall of yeast
Zymolyase-20T is an enzyme preparation from a submerged culture of
Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is a lyophilized powder prepared by salting out from the culture fluid with ammonium sulfate and contains 20,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells is ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.
