General description
TRI reagent® also known as TRIzol, is a
monophasic solution of phenol and guanidinium isothiocyanate. It has a
potential, to simultaneously solubilize biological material and denatures
protein. It is widely used for deproteinizing RNA.[1] This improved
version of the single-step total RNA isolation reagent developed by Chomczynski
allows a quick, economical, and efficient isolation of total RNA from different
biological samples. This product is pure (A260/A280 ~1.8)
and can be directly used in Northern blotting. Simultaneous isolation of DNA,
RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100
mg tissue or 107 cells.
Application
TRI Reagent is an improved version of the single-step total
RNA isolation reagent developed by Chomczynski. The RNA isolation method based
on this reagent is widely used and proven for RNA applications. It is ideal for
quick, economical, and efficient isolation of total RNA or the simultaneous
isolation of RNA, DNA, and proteins from samples of human, animal, plant,
yeast, bacterial, and viral origin.
TRI Reagent® has been used for isolating RNA
from various types of cells or tissues.[2][3][4] It may also be used for
processing tissues, cells cultured in monolayer, or cell pellets.
Features and Benefits
- Easily
scalable RNA isolation
- Works
with many sources: human, plant, yeast, bacterial, or viral
- Better
yields than traditional guanidine thiocyanate/cesium chloride methods
Legal Information
TRI Reagent is a registered trademark of Molecular Research
Center, Inc.
