General description
Azocasein is a chromogenic derivative of
casein.[1] Protease degrades azocasein to yield TCA-soluble azopeptides
with high UV-absorbance. This azocasein assay is widely employed to estimate
the protease production by bacterial fermentation on synthetic substrates from
glucose and inorganic salts.[2]
Application
Azocasein has been used as a substrate for determination of
protease activity.[3][4][5]
Azocasein is a nonspecific protease substrate. Hydrolysis of
the casein releases the azo dye into the media where it is detected by
absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce
amyloid A amylooidosis in experimental animals.
Other Notes
View more information on azocasein
protease assay at www.sigma-aldrich.com/enzymeexplorer
