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Supelco® N,N′-Bis(acryloyl)cystamine BioReagent, suitable for electrophoresis

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MT-CM-A4929-5G


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Empirical Formula (Hill Notation):  C10H16N2O2S2

CAS Number:  60984-57-8

Molecular Weight:  260.38

EC Number:  262-546-8

MDL number:  MFCD00036225

UNSPSC Code:  12352116

PubChem Substance ID:  24890926

NACRES:  NA.25

product line  BioReagent

Quality Level  200

Form  powder

Suitability  suitable for electrophoresis

storage temp.  −20°C

SMILES string  C=CC(=O)NCCSSCCNC(=O)C=C

InChI  1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)

InChI key  DJVKJGIZQFBFGS-UHFFFAOYSA-N

Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.

BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.