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Sigma-Aldrich®Acrylamide Molecular Biology, ≥99% (HPLC)

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MT-CM-A9099-25G

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Linear Formula:  CH2=CHCONH2

CAS Number:  79-06-1

Molecular Weight:  71.08

Beilstein:  605349

EC Number:  201-173-7

MDL number:  MFCD00008032

UNSPSC Code:  41105319

eCl@ss:  39031205

PubChem Substance ID:  24891226

NACRES:  NA.31

Grade  Molecular Biology

Quality Level  200

vapor density  2.45 (vs air)

vapor pressure  0.03 mmHg ( 40 °C)

Assay  ≥99% (HPLC)

Form  crystals

impurities

≤0.005% Acrylic acid content

≤0.005% Water insolubles

Conductivity  ≤10 μmho (35% solution)

Bp  125 °C/25 mmHg (lit.)

Mp   82-86 °C (lit.)

Solubility  water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm

Mg: ≤3 ppm

heavy metals (as Pb): ≤5 ppm

foreign activity  DNase, RNase, protease, none detected

SMILES string  NC(=O)C=C

InChI  1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key  HRPVXLWXLXDGHG-UHFFFAOYSA-N

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide. Acrylamide is a water-soluble monomer, which forms a transparent stable insoluble gel. Bis-acrylamide is a cross-linking agent.[1][2]

Application

Acrylamide has been used:


  • in polyacrylamide gel electrophoresis[3][4]
  • in the preparation of cleared gel samples[5]
  • to determine the effect of L-cysteine on acrylamide-induced testicular toxicity[6]