ApsalagenTM Hot Start PCR Master Mix is a perfect choice for a fast reaction setup that reduces
the time required for calculation and pipetting and eliminates the need for buffer optimization.
It is designed for low-background, high-throughput PCR of 0.2–5 kb DNA targets.
The 2x Hot Start PCR Master Mix contains pure Hot Start Taq DNA Polymerase, extremely highquality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade
water are added.
The Hot Start Taq DNA Polymerase is inactive during reaction setup due to the bound antibody,
which is quickly released at elevated temperatures, ensuring the enzyme is active only during
PCR. There is no need for prolonged heating or denaturation steps. The hot start minimizes
primer–dimers and mispriming.
The optional use of 5× PCR Enhancer improves PCR results in many cases, including impure
template or low template abundance.
